Diagnostic Interests and Goals
Standard PCR (polymerase chain reaction) is the ability to amplify a specific gene with the use of specially designed primers. Any standard PCR is run with a known negative and known positive sample to ensure each reaction is run properly. If a sample appears suspect, it is automatically submitted for sequencing to confirm what species is being detected. After sequence confirmation is completed, a result is sent out. Click here to access the PCR zoo submission form.
Quantitative (qPCR aka Real Time) PCR is the ability to detect the amount of a specific virus/gene present in a sample. This is especially useful when determining how much treatment should be given, as well as seeing if the treatment has worked over a period of time.
Serology is only offered for Paramyxovirus Group B. Plasma is tested looking specifically for antibodies present in this group, not active infection. If a snake were to show a positive titer, it is suggested to retest that animal by PCR to determine if there is an active infection.
Interpretation of any results needs to be done in appropriate clinical context. To reduce misinterpretation and misuse of results, we only accept samples submitted by licensed veterinarians. The submitting vet or institution will also be responsible for payment.
Current DNA PCR
Click here for the submission protocol for DNA
- 16S Bacterial
- Acanthocephalan
- Adenovirus
- Anaplasma
- Babesia
- Barcoding
- Blastocystis
- Bocavirus
- Botulinium
- Cestode
- Chlamydiophila
- Circovirus
- Coccidia
- Consenus Polymerase
- Coxiella
- Cryptosporidium
- Cyanobacteria
- Eimeridiia
- Fungal
- Fusarium
- Haemosporidia
- Hemogregarian
- Hepadnavirus
- Herpesvirus
- Histoplasmosis
- Isospora
- Lagenidium
- Lawsonia
- Leishmania
- Microsporidia
- Mycobacteria
- Nematode
- Papillomavirus
- Parvovirus
- Pentastomid
- Polyomavirus
- Poxvirus
- Sarcocystis
- Trematode
Current RNA PCR
Click here for the submission protocol for RNA
- Astrovirus
- Bornavirus
- Calicivirus
- Coronavirus
- Enterovirus
- Inclusion Boid Disease (IBD)
- Nido/Torovirus
- Paramyxovirus
- Picornavirus
- Reovirus
- Rhabdovirus
- Sunshinevirus
Current qPCR
- Agamid Adenovirus in Bearded Dragons
- Anaplasma Gro
- Anaplasma Suc
- Box Turtle Adenovirus
- Box Turtle Herpesvirus
- Crypto serpentis
- CSL Adenovirus
- Finch Polyomavirus
- Intranuclear Coccidiosis
- Pythium Insidiosum
- Tortoise Herpesvirus 2
- Tortoise Picornavirus