Columbian black-tailed deer liver cells (OHH1.Li) infected with mammalian orthoreovirus (MRV). Viral antigens (red) were detected using rabbit polyclonal antiserum directed against Type 3 MRV and a Cy5-labeled goat anti-rabbit IgG secondary antibody. Actin filaments (green) were detected with a phalloidin probe conjugated with an Alexa Fluor 488 dye. Cell nuclei (blue) are counterstained with DAPI. Note the formation of condensed, globular viral factories (red) adjacent to cell nuclei which represent distinct areas of viral replication and assembly in the cytoplasm.
In addition to research, our lab also conducts viral diagnostics on a case-by-case basis. Specifically, we perform virus isolation and virus identification from suspect disease cases of wildlife or zoological animals. Additional serological assays such as plaque-reduction neutralization tests or virus microneutralization may be available. Please contact Andrew Allison (aallison1@ufl.edu) to inquire about possible testing and for appropriate shipping guidelines.
After consultation, samples should be sent priority overnight to the address below.
Shipping address:
Andrew Allison
2015 SW 16th Ave
CDPM Dept, VAB Room V2-209
College of Veterinary Medicine
University of Florida
Gainesville, FL 32610