Cleverson De Souza

Cleverson De Souza,

Associate Professor – Clinical Pathology

Department: Department of Comparative, Diagnostic & Population Medicine
Business Phone: (352) 294-4153
Business Email:

About Cleverson De Souza

Dr. Souza obtained his DVM in 1999 from Universidade Federal do Parana, Brazil, followed by a two-year residency program in Clinical Pathology in São Paulo (UNESP-Botucatu), Brazil. Subsequently, he pursued a Master of Science in Clinical Pathology at Universidade Federal de Santa Maria in Rio Grande do Sul, Brazil. After gaining experience as a veterinary clinical pathologist at Universidade de Passo Fundo in Rio Grande do Sul, Dr. Souza joined Dr. Douglas J. Weiss’s lab at the University of Minnesota to research the molecular pathogenesis of Mycobacterium paratuberculosis. He also completed an accelerated residency in veterinary Clinical Pathology at the same institution and achieved board certification through the American College of Veterinary Pathologists (ACVP). Between 2009 and 2012, Dr. Souza shared his expertise in general pathology with MD and DDS students at Brazilian universities such as Universidade de Fortaleza, UniChristus, and Universidade Federal do Ceara. Over the following decade (2013-2023), he held a tenured associate professor position at Washington State University, where he worked as a Clinical Pathologist. Currently, Dr. Souza contributes as an Associate Professor in the Department of Comparative, Diagnostic & Population Medicine at the University of Florida, dedicating his efforts to service, teaching, and research.

Teaching Profile

Courses Taught
VEM5221 Intro Clinic Pathol
VEM5854 Clinical Pathology
VEM5855 Clkship Clin Pathol
Teaching Philosophy
My teaching philosophy is based on evidence-based learning strategies that foster long-term retention and mastery. I believe in creating an engaging environment that encourages active participation, reflection, and self-directed learning. Key elements of my approach include: -Designing active learning experiences through group discussions, problem-solving exercises, and collaborative projects -Incorporating spaced practice and interleaving by revisiting and integrating previously covered material and related concepts -Utilizing retrieval practice through low-stakes quizzes, self-assessment exercises, and peer teaching opportunities -Promoting a growth mindset by emphasizing effort, persistence, and learning from mistakes -Encouraging metacognitive awareness through self-assessment and reflection on learning strategies -Providing timely, specific, and constructive feedback to guide student growth and goal-setting By integrating these principles, I aim to empower students to take ownership of their education, develop lifelong learning skills, and achieve enduring understanding and mastery.

Board Certifications

  • Clinical Pathology
    American College of Veterinary Pathologists (ACVP)

Research Profile

Numerous focus areas are being pursued to advance the field, encompassing basic, clinical, and educational research. Key areas of investigation include:

Basic research: Developing peptide-based vaccines against Mycobacterium Paratuberculosis (MAP) in bovine, Functional Characterization of Tumor Associate Macrophages in Dogs

Clinical research: Utilizing nanomaterials and dendritic cells to enhance immune responses against malignant tumors in dogs, Validating new monoclonal antibodies for detecting lymphoma in canine patients, Applying machine learning and deep learning techniques to aid the diagnosis of lymphoma in dogs, Studying aspects of the immune response in exotic animal species

Areas of Interest
  • Cancer Immunology and Immunotherapy
  • Clinical pathology of mammals
  • Vaccine immunology
  • Veterinary Education


Preliminary Evaluation of an Autologous Dendritic Cell Vaccine Using Nanoparticle Technology for the Treatment of Canine Malignant Melanoma
American Journal of Biomedical and Life Sciences. 9(1):84-96 [DOI] doi: 10.11648/j.ajbls.20210901.21.
Salmon poisoning disease in dogs: clinical presentation, diagnosis and treatment.
Brazilian journal of veterinary medicine. 44 [DOI] 10.29374/2527-2179.bjvm004822. [PMID] 36475066.
A peptide-based vaccine for Mycobacterium avium subspecies paratuberculosis.
Vaccine. 37(21):2783-2790 [DOI] 10.1016/j.vaccine.2019.04.040. [PMID] 31003915.
A Mycobacterium avium subsp. paratuberculosis relA deletion mutant and a 35 kDa major membrane protein elicit development of cytotoxic T lymphocytes with ability to kill intracellular bacteria.
Veterinary research. 49(1) [DOI] 10.1186/s13567-018-0549-3. [PMID] 29941017.
A nano particle vector comprised of poly lactic-co-glycolic acid and monophosphoryl lipid A and recombinant Mycobacterium avium subsp paratuberculosis peptides stimulate a pro-immune profile in bovine macrophages.
Journal of applied microbiology. 123(1):54-65 [DOI] 10.1111/jam.13491. [PMID] 28502107.
In Vitro Evaluation of the Biological Responses of Canine Macrophages Challenged with PLGA Nanoparticles Containing Monophosphoryl Lipid A.
PloS one. 11(11) [DOI] 10.1371/journal.pone.0165477. [PMID] 27835636.
Blocking the mitogen activated protein kinase-p38 pathway is associated with increase expression of nitric oxide synthase and higher production of nitric oxide by bovine macrophages infected with Mycobacterium avium subsp paratuberculosis.
Veterinary immunology and immunopathology. 164(1-2):1-9 [DOI] 10.1016/j.vetimm.2015.01.007. [PMID] 25700780.
Mycobacterium avium Subspecies paratuberculosis Recombinant Proteins Modulate Antimycobacterial Functions of Bovine Macrophages.
PloS one. 10(6) [DOI] 10.1371/journal.pone.0128966. [PMID] 26076028.
Mannosylated lipoarabinomannans from Mycobacterium avium subsp. paratuberculosis alters the inflammatory response by bovine macrophages and suppresses killing of Mycobacterium avium subsp. avium organisms.
PloS one. 8(9) [DOI] 10.1371/journal.pone.0075924. [PMID] 24098744.
Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
Journal of leukocyte biology. 83(1):48-55 [PMID] 17913973.
Effects of nuclear factor-kappaB on regulation of cytokine expression and apoptosis in bovine monocytes exposed to Mycobacterium avium subsp paratuberculosis.
American journal of veterinary research. 69(6):804-10 [DOI] 10.2460/ajvr.69.6.804. [PMID] 18518662.
Review paper: modulation of mononuclear phagocyte function by Mycobacterium avium subsp. paratuberculosis.
Veterinary pathology. 45(6):829-41 [DOI] 10.1354/vp.45-6-829. [PMID] 18984786.
Role of cell membrane receptors in the suppression of monocyte anti-microbial activity against Mycobacterium avium subsp. paratuberculosis.
Microbial pathogenesis. 44(3):215-23 [PMID] 18079089.
Cell membrane receptors on bovine mononuclear phagocytes involved in phagocytosis of Mycobacterium avium subsp paratuberculosis.
American journal of veterinary research. 68(9):975-80 [PMID] 17764412.
Role of the MAPK(ERK) pathway in regulation of cytokine expression by Mycobacterium avium subsp paratuberculosis-exposed bovine monocytes.
American journal of veterinary research. 68(6):625-30 [PMID] 17542695.
Role of the mitogen-activated protein kinase pathway in the differential response of bovine monocytes to Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
Microbes and infection. 9(14-15):1545-52 [PMID] 18035573.
Mitogen activated protein kinase p38 pathway is an important component of the anti-inflammatory response in Mycobacterium avium subsp. paratuberculosis-infected bovine monocytes.
Microbial pathogenesis. 41(2-3):59-66 [PMID] 16716561.
Mucosal immune response in cattle with subclinical Johne’s disease.
Veterinary pathology. 43(2):127-35 [PMID] 16537930.
Regulation by Jun N-terminal kinase/stress activated protein kinase of cytokine expression in Mycobacterium avium subsp paratuberculosis-infected bovine monocytes.
American journal of veterinary research. 67(10):1760-5 [PMID] 17014329.
A critical role of interleukin-10 in the response of bovine macrophages to infection by Mycobacterium avium subsp paratuberculosis.
American journal of veterinary research. 66(4):721-6 [PMID] 15900955.
Expression of interleukin-10 and suppressor of cytokine signaling-3 associated with susceptibility of cattle to infection with Mycobacterium avium subsp paratuberculosis.
American journal of veterinary research. 66(7):1114-20 [PMID] 16111147.


Residency in Veterinary Clinical Pathology
2008-2009 · University of Minnesota, College of Veterinary Medicine, St Paul, MN
2003-2008 · University of Minnesota, St Paul, MN
MS Immunology track
2001-2002 · Federal University of Santa Maria, RS, Brazil
Residency in Veterinary Clinical Pathology
2000-2001 · UNESP, Botucatu, SP, Brazil
Doctor of Veterinary Medicine
1994-1999 · Federal University of Parana, PR, Brazil

Contact Details

(352) 294-4153
Business Street:
2015 SW 16TH AVE