Shiyu Li

Directory Profile

Lab: Dr. Aline Rodrigues Hoffman’s Lab

Title: Lab Technician III

Lab Skills & Training: Skilled at cell culture, including suspended and adherent cell lines (e.g. stem cells, cancer cells , renal cells, Human Aortic Smooth Muscle Cells (HAoSMC), Human umbilical vein endothelial cells (HUVECs) et al.); Skilled in various molecular and cell biology techniques including Western blotting, ELISA, immunofluorescence, Flow cytometry, migration assay; DNA/RNA/protein extraction from blood/cell/tissue; Real-time PCR, gene arrays.

Equipment Proficiency: BD Biosciences Flow Cytometer, BD FACSCanto Systems, BD LSR Flow Cytometer systems, AutoMACS separator, Lionheart FX Automated Microscope, Biotek Synergy Microplate Reader

Research Interests:

  • Cardiovascular Cell Therapy Research Network (CCTRN).
  • Mechanisms underlying vasodilation and increased arterial compliance during normal pregnancy with emphasis on the hormone relaxin; role of relaxin and pregnancy in bone marrow derived progenitor cell mobilization and function.
  • Epigenetics and control of epithelial sodium channel expression in collecting duct.
  • Generate and culture human embryonic stem cells.