Lab: Dr. Aline Rodrigues Hoffman’s Lab
Title: Lab Technician III
Lab Skills & Training: Skilled at cell culture, including suspended and adherent cell lines (e.g. stem cells, cancer cells , renal cells, Human Aortic Smooth Muscle Cells (HAoSMC), Human umbilical vein endothelial cells (HUVECs) et al.); Skilled in various molecular and cell biology techniques including Western blotting, ELISA, immunofluorescence, Flow cytometry, migration assay; DNA/RNA/protein extraction from blood/cell/tissue; Real-time PCR, gene arrays.
Equipment Proficiency: BD Biosciences Flow Cytometer, BD FACSCanto Systems, BD LSR Flow Cytometer systems, AutoMACS separator, Lionheart FX Automated Microscope, Biotek Synergy Microplate Reader
- Cardiovascular Cell Therapy Research Network (CCTRN).
- Mechanisms underlying vasodilation and increased arterial compliance during normal pregnancy with emphasis on the hormone relaxin; role of relaxin and pregnancy in bone marrow derived progenitor cell mobilization and function.
- Epigenetics and control of epithelial sodium channel expression in collecting duct.
- Generate and culture human embryonic stem cells.